Project
101-F1-F2

Part:BBa_K2491025:Design

Designed by: Philippe Hansen-Estruch   Group: iGEM17_CCA_San_Diego   (2017-10-10)


Combination of Part 1 and Part 2 of Fluorene Degradation - 101


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 3149
    Illegal NheI site found at 3172
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5231
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5049
    Illegal AgeI site found at 6061
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 403
    Illegal BsaI.rc site found at 1515
    Illegal SapI.rc site found at 5592


Design Notes

Read F1 and F2 (weak promoter) as this sequence is just the ligation of them.


Source

Synthetic Genes based on Terrabacter sp. DBF63

References